Coding

Part:BBa_K1152000:Design

Designed by: Tania Christiansen   Group: iGEM13_Heidelberg   (2013-09-16)

TycA_deltaCom Module from Tyrocidine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2518


Design Notes

This is just the coding sequence. We sequenced with VR and VF2 primer, no mutations were found (http://2013.igem.org/File:Heidelberg_RFC10_TycASeq.zip). You can use it to combine it with other modules of e.g. the Tyrocidine cluster for the construction of a new non-ribosomal peptide. Go to our wiki: http://2013.igem.org/Team:Heidelberg/Parts File:Heidelberg RFC10 TycASeq.zip

Source

Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835

References